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ELISA Training, May 2025
- Date
- 23.05.25
- Start time
- 10:00
- End time
- 13:00
- Where
- Heinz Wolff building, Brunel University, Kingston Ln, London, Uxbridge
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* * This course contributes to the Royal Society of Biology's Technical Skills Certificate
Overview
The world is full of pathogens that infect us and cause disease. Often these can be grown in culture for identification purposes, but with some pathogens it is difficult to collect samples, they are difficult to grow and diagnosis can take a long time. Therefore, other diagnostic techniques must be used in clinical setting to allow rapid and cost-effective detection and identification. One such technique routinely used is the enzyme-linked immunosorbent assay (ELISA). This is an immunological based assay that can be used to measure antibodies, antigens, proteins and glycoproteins associated with the pathogen in biological samples. On this practical course you will learn about the different types of ELISA, how they work, and use a working example to detect if some patients have an immune response to Aspergillus fumigatus.You will learn both the Indirect and Sandwich ELISA techniques, understand the pathogen Aspergillus fumigatus, and how it can induce a hyperactive immune response (allergic response) to the presence of this pathogen.
Aims
The broad aims of the course are to:- Provide the foundations of what ELISA is and why it is important for patient diagnosis.
- Introduce the equipment and techniques associated with undertaking different types of ELISA.
- Provide practical application of undertaking ELISA tests.
- Guide on the analysis of results and what the results mean.
Who is the Course For?
Biological and medical scientists who wish to learn the ELISA technique, and how to refine and apply it. No prior knowledge of the ELISA technique will be required, and the purpose of the course is to offer a practical point of access to this technique in a practical user-friendly form.Learning Outcomes
Knowledge and understanding:- Understand the principles of the enzyme-linked immunosorbent assay (ELISA).
- Learn about Aspergillus fumigatus and how it can cause both infections and allergic responses.
- Learn the different types of ELISA (Indirect and Sandwich).
- The practical applications of ELISA.
- Understand how the data is analysed and what it means.
Course Tutor
The course will be run by Dr Ashley Houlden. Ashley is a microbial ecologist, with research interests in microbial community (host-microbiome) structure and function. He has studied soil microbial ecology relating to fungi diseases in crops, before moving into research on the medical microbiome.Ashley is a co-investigator in the “Organ-on-a-Chip” interdisciplinary research group at Brunel University London. This research group focuses on developing in vitro models of the breast, vagina, ovary and placenta, with a view to their use in pre-clinical trials of new drugs.
Ashley is currently a Lecturer in the Division of Biosciences, College of Health, Medical and Life Sciences at Brunel University London. He teaches at second year undergraduate and at MSc level, and has also been involved in providing lab “taster” sessions for secondary school children and our BSc Biomedical Science course applicants.
Certification and Continuing Professional Development (CPD)
A certificate of attendance will be provided after the event.We evaluate all of our training events, to make sure that we maintain a high quality of training. This event has been approved by the Royal Society of Biology for purposes of CPD and can be counted as 9 CPD points.
Professional Registers
This course supports registers competencies and has been identified as supporting competency development for: Registered Science Technician (RSciTech).Fees
Non-members - £440 + 20% VAT
Get in touch with training@rsb.org.uk to access these rates:
- Members of Member Organisations, SCAS members - £330 + 20% VAT
- Non-members who have completed a membership application and made payment - £220 + 20% VAT.
If you or your organisation wishes to book staff in bulk for this course, please get in touch.
Further information
Lunch will not be provided.For further information about the course please contact Raksha Gohel, Training and Registers Officer at training@rsb.org.uk or on 020 3925 3449.